As a part of EGEE-III NA3 activities, a one day user-oriented training event has been organized by Scientific Computing Laboratory at the Faculty of Sciences, University of Novi Sad on February 6, 2009. The aim of the training was to provide users with basics of high performance and Grid computing, and gLite middleware architecture and services. The participants are also given introduction to EGEE-III, and an overview of regional Grid projects and national Grid activities. Basic usage of the gLite middleware is presented in hands-on session with demonstrations. More than 30 researches and students mostly from Faculty of Sciences, University of Novi Sad participated in this event.